Detection of Oxacillin Resistance in Staphylococcus aureus: Comparison of Phoenix Oxacillin and Cefoxitin MICs, MicroScan Oxacillin MIC, Oxacillin and Cefoxitin Disk Diffusion, and mecA Gene Detection

نویسندگان

  • H. SALIMNIA
  • W. J. BROWN
چکیده

BACKGROUND. Phenotypic tests for detection of mecA containing staphylococci have been challenged. Cefoxitin (FOX) disk diffusion (DD) has been reported to more accurately predict mecA gene presence than oxacillin (OX) DD. The Phoenix instrument rapidly provides identification and susceptibility data and it is of interest to determine if an experimental panel containing OX and FOX could accurately detect OX resistance.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Detection Of Methicillin Resistance In Staphylococcus Aureus By Disk Diffusion And PCR Methods

  Background and Objective: Methicillin resistance in Staphylococcus aureus is an increasingly important clinical problem. A chromosomal gene, mecA, mediates resistance to penicillinase-resistant penicillins such as methicillin and oxacillin in Staphylococcus aureus. We evaluated the validity of disk diffusion test by using oxacillin, methicillin an...

متن کامل

Evaluation of Cefoxitin Disk Diffusion Test for Routine Detection of Methicillin-resistant Staphylococcus Aureus

Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community acquired infections. Detection of MRSA in laboratories is very important for treatment and appropriate infection control. The aim of this study was to evaluate cefoxitin disk diffusi...

متن کامل

Differentiationo Methicillin-Resistant and Methicillin-Susceptible Staphylococcus Aureus and Coagulase-Negative Staphylococci using Cefoxitin

Detection of the mecA gene or PBP2a is considered the gold standard for detecting mecA-mediated methicillin resistance in staphylococci. Among available phenotypic methods, the Clinical and Laboratory Standards Institute (CLSI) has introduced the cefoxitin disk diffusion (DD) test for predicting the presence of mecA in S. aureus and CoNS, which is preferred over the oxacillin DD test. Automated...

متن کامل

Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)

Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...

متن کامل

Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group.

Staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. Human infections with S. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. Oxacillin and ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2006